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Research Project:
Evaluation of Ppv Resistance in Native, Naturalized, and Ornamental Prunus in the U.S. with Emphasis on the Ne and Mid-Atlantic States
Location:
Appalachian Fruit Research Laboratory
Innovative Fruit Production, Improvement and Protection
Project Number: 1931-21000-011-04
Project Type:
Reimbursable
Start Date: Oct 01, 2002
End Date: Sep 30, 2006
Objective:
The objective of this work is to evaluate the susceptibility and symptom expression of a wide range of Prunus species native, naturalized, and ornamental, inoculated with plum pox virus strain D, Pennsylvania isolate.
Approach:
Six plants of each of approximately 30 species tested will be inoculated by bud-grafting with budwood from trees infected with the Pennsylvania D strain of plum pox virus (PPV). One month following inoculation, trees will be exposed to an artificial 2-month cold dormancy period in a cold chamber. Following dormancy, inoculated trees will be moved to the greenhouse and symptoms of PPV will be evaluated as trees grow. ELISA and PCR will be used to critically evaluate infections symptoms; when apparent will be photographed.
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