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Research Project:
ENHANCEMENT OF BLUEBERRY AND STRAWBERRY THROUGH ANALYSIS AND MODIFICATION OF THE PLANT GENOME
Location:
Fruit
Project Number: 1275-21000-157-00
Project Type:
Appropriated
Start Date: Jun 13, 2002
End Date: Aug 31, 2006
Objective:
Identify genes/molecular markers of horticultural significance in small fruits to make them available for transformation and marker-assisted breeding of small fruit cultivars, and to develop optimum tissue culture and gene transfer technologies for introducing disease resistance, cold tolerance and chilling requirement determining genes into small fruits.
Approach:
Studies will focus on: 1) isolating and characterizing the dehydrin gene family of blueberry, as well as search for new chilling/cold responsive genes using a genomic approach, 2) mapping chilling/cold responsive genes and other candidate genes in blueberry and determine if any map to QTLs that control chilling requirement or cold hardiness, 3) identifying disease resistance genes in blueberry and strawberry and molecular markers linked to these genes, as well as disease resistance genes from other sources that can be used for genetic engineering of small fruit crops, 4) developing optimum conditions for gene delivery, selection and regeneration of several important blueberry and strawberry cultivars developed for the eastern United States, 5) utilizing transformation technologies to introduce cold tolerance genes into elite blueberry cultivars and disease resistance genes into both blueberry and strawberry cultivars, and 6) developing in vitro disease resistance screening methods for elite blueberry and strawberry cultivars and using this technology to identify genetically engineered and/or tissue culture modified germplasm with increased levels of disease resistance. BSL-1 Recertified 8/16/01.
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