Project Number: 1932-61000-002-02
Project Type: Specific C/A

Start Date: Feb 01, 1999
End Date: Jan 31, 2004

Objective:
To identify the most appropriate molecular biology technology for monitoring global gene expression in root-colonizing bacteria important to agriculture.

Approach:
Bacterial cells will be subjected to stressful soil conditions via soil solutions for various times. Gene expression will be monitored by either nucleic acid (ex., differential display) or protein (ex., high resolution gel electrophoresis)-based techniques. Resolution, speed and accuracy will be assessed for use with rhizobial and pseudomonal bacteria for identifying genes and/or their products responsive to starvation and acidic soil conditions.

Project Team

Staley, Thomas David Yelton - Professor Of Microbiology (304)256-2821

Project Annual Reports

FY 2003   FY 2002   FY 2001   FY 2000   FY 1999

Related National Programs

Soil Resource Management (202)   Plant Biological and Molecular Processes (302)