Project Number: 5302-22000-005-08
Project Type: Specific C/A

Start Date: Sep 25, 2003
End Date: Aug 31, 2004

Objective:
To develop and test protocols for in situ hybridization histology of Xylella fastidiosa in infected grape tissues.

Approach:
Two established methods will be modified and blended to achieve DNA-DNA in situ hybridization of Xylella fastidiosa bacteria in sectioned grape tissue, for epifluorescent and confocal visualization of bacteria in planta. RNA-RNA hybridization of sectioned plant material, and DNA-DNA hybridization of unsectioned, cleared and macerated cells will be used. Other, literature-based methods will also be consulted and portions added as needed or appropriate. Documents SCA with U. of MO.

Project Team

Backus, Elaine Quisheng Song - Assistant Professor, Entomology Department 559-596-2925

Related National Programs

Plant Diseases (303)   Crop Protection & Quarantine (304)