Project Number: 5442-22000-038-00
Project Type: Appropriated

Start Date: Jun 06, 2004
End Date: Oct 31, 2005

Objective:
Develop methods for short and long-term cold storage of insects such as Perillus, tephritid fruit flies, and glassy-wing sharpshooter used in area-wide sterile release and biocontrol programs; and for insects used in fundamental high impact research projects which generate or acquire numerous valuable genetic strains.

Approach:
Design short-term storage techniques by determining developmental and stage specific cold tolerance and susceptibility to manipulation of dormancy; Devise cryogenic procedures for long-term storage in liquid nitrogen by determining chilling tolerance, membrane permeability, cryoprotectant and post-storage recovery requirements.

Project Team

Leopold, Roger

Related National Programs

Plant, Microbial & Insect Genetic Res., Genomics, & Genetic Improv. I (301)   Crop Protection & Quarantine (304)

Related Projects

CRYOPRESERVATION OF FRUIT FLY (TEPHRITIDAE) SPERM AND IN VITRO FERTILIZATION    LONG-TERM COLD STORAGE OF CARIB AND MEDFLY GERMPLASM    COLD STORAGE OF PARASITIZED AND UNPARASITIZED EGGSOF THE GLASSY-WINGED SHARPSHOOTER, H. COAGULATA    COLD STORAGE OF PARASITIZED EGGS OF THE GLASSY-WINGED SHARPSHOOTER, H. COAGULATA