Project Number: 1935-12000-007-02
Project Type: Specific C/A

Start Date: Dec 16, 2002
End Date: Dec 31, 2004

Objective:
To determine in what forms(s) nitrogen is exchanged between the mycorrhizal fungi and plant host using gene expression experiments. Establish the relationship between carbon uptake by the fungus and nitrogen uptake by the host.

Approach:
Isolate both fungal and host tissue from RIT-DNA mycorrhizal transformed roots colonized with Glomus intraradices under various conditions during the symbiotic life cycle. Using quantitative PCR to determine real time expression of urease, ornithine amino transferase and arginase genes at different phases of the life cycle. Establish the 15N labeling patterns in amino acids in fungal protein to determine the metabolic pathways used for the assimilation of nitrogen pand transfer to the host.

Project Team

Pfeffer, Philip Peter Lammers - Professor 215-233-6469

Project Annual Reports

FY 2003

Related National Programs

Soil Resource Management (202)   Plant Biological and Molecular Processes (302)