Project Number: 1275-22000-199-00
Project Type: Appropriated

Start Date: Jan 10, 2003
End Date: Jan 09, 2008

Objective:
1. Develop a phylogeny for Hypocrea, Trichoderma and related fungi with emphasis on two cryptic species-complexes, Trichoderma viride, the type species, and T. hamatum, the most commonly isolated group. 2. Develop a characterization and identification scheme for Hypocrea, Trichoderma and related groups used in the biological control of plant pathogenic fungi on specific commodities.

Approach:
Fresh specimens of Hypocrea and Trichoderma will be collected by the SY from around the world especially the sexual state from which ascospore isolates produce the Trichoderma state in culture. The morphology of both sexual and asexual states will be characterized using an image analysis system. All descriptive and potentially diagnostic structures are measured. Molecular sequences are obtained for each isolate. Phylogenetic analyses will use the ITS of rDNA as well as protein coding genes such as introns of the translation-elongation factor 1-alpha, RBP2, calmodulin and actin. Species concepts will be defined using both molecular and morphological data. A taxonomy will be developed and published as a taxonomic monograph. Isolates sent for identification will be studied using methods similar to those applied to isolates studied for the monographic accounts. New species not included in monographic studies will be described and illustrated in order to facilitate their use and development as biological control agents. An on-line, interactive identification system will be developed. For each species numerous graphics will be added to illustrate all morphological and cultural characteristics.

Project Team

Samuels, Gary

Project Annual Reports

FY 2003