Project Number: 3620-22410-008-02
Project Type: Trust

Start Date: Jun 01, 2004
End Date: May 30, 2007

Objective:
The objective of this collaboration is the optimization of a commercial method for producing and formulating infective microsclerotial propagules of the bioherbicide Mycoleptodiscus terrestris for control of hydrilla, a serious aquatic weed in the Southern United States.

Approach:
The production and stabilization of infective microsclerotia of M. terrestris has been accomplished using submerged, liquid culture fermentation. Optimization of the production and harvesting of M. terrestris microsclerotia will continue with the goal of maximizing biocontrol efficacy. In addition, formulation studies with M. terrestris microsclerotia will focus on improving the biocontrol efficacy of these propagules while maintaining adequate shelf-life. Formulation goals will be 1) to identify chemistries, compositions, or physical forms that enhance the adherence of M. terrestris microsclerotia to hydrilla; 2) to identify nutrient-containing formulates that improve the biocontrol efficacy of M. terrestris microsclerotia by increasing infectivity (production of infective spores, enhanced microsclerotia germination rate, etc.); and 3) to develop a formulated product that is suitable for commercial application. Bioassays to determine the biocontrol efficacy and overall fitness of M. terrestris formulates derived from these studies will be performed on hydrilla by SePRO. Promising formulation of M. terrestris will be produced and tested in field trials as necessary. Field trial studies for hydrilla control with M. terrestris formulations will be designed and directed by employees of SePRO.

Project Team

Jackson, Mark

Related National Programs

Crop Protection & Quarantine (304)   Quality and Utilization of Agricultural Products (306)