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Display category headings
Research Project:
Enhancement of Elite Small Grains Germplasm Utilizing Tissue Culture and Molecular Approaches
Location:
Small Grains and Potato Germplasm Research
Project Number: 5366-21000-024-01
Project Type:
Specific C/A
Start Date: Sep 30, 1999
End Date: Aug 31, 2004
Objective:
Evaluation and enhancement of oat, barley, wheat, and other small grains germplasm, specifically involving the study of the effect of genotype on tissue culture performance leading to the enhanced utilization of tissue culture in small grains research, enhanced plant regeneration from tissue culture, and expanded plant transformation research. Characterization of diverse small grains germplasm, with emphasis on molecular approaches, especially involving protein and DNA characterization.
Approach:
Determine the effect of genotype on cereal protoplast and tissue culture performance leading to the enhanced utilization of tissue culture in small grains research. Characterize small grains germplasm, with emphasis on molecular approaches, including the study of phylogenetic allozyme and other protein differences among wild and cultivated accessions within a species. The tissue culture, plant regeneration, plant transformation, and molecular characterization phases of the effort will involve a wide array of National Small Grains Collection (NSGC) and other elite germplasm. The project will specifically involve the evaluation of progeny from crosses and backcrosses, with selective evaluation for somatic embryogenesis in callus culture and the development of a suspension culture system for oats. Project participants will include ARS and state researchers as well as Alabama A&M; University students. Documents SCA with Alabama A&M.;
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Project Team |
![](/peth04/20041119141951im_/http://www.ars.usda.gov/images/list_but_3.gif) |
Bonman, John
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![](/peth04/20041119141951im_/http://www.ars.usda.gov/images/list_but_3.gif) |
Govind Sharma - Chairman Aamu Plant And Soil Science (208)397-4162 |
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Related National Programs |
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