Project Number: 5430-21000-005-12
Project Type: Reimbursable

Start Date: Oct 01, 2002
End Date: Sep 30, 2004

Objective:
The first objective of the proposed research is to use PCR-based microsatellite markers to develop an anchored framework map of a large recombinant inbred (RI) population of winter wheat from a cross between "Rio Blanco" and "Idaho 444." The second objective of the proposed research is to identify genomic regions associated with the highly robust resistance to dwarf bunt (Tilletia controversa Kuhn in Rabenh) in this population.

Approach:
The revised objective is to identify 200+ genetic markers that are polymorphic among the parents and useful for mapping. Polymorphism of Rio Blanco and Idaho 444 will be evaluated using publicly available SSR primer pairs. Six random RI lines will be included in this screening to verify utility of polymorphism. A collection of microsatellite primer pairs that are labeled for use on the ABI 3100 and ABI 3700 DNA fragement analysers are maintained in the laboratory of Dr. Gina Brown-Guedira. Preliminary data from microsatellite markers in use in the PI's laboratory indicates that approximately 36% of primer pairs will produce useful polymorphisms in the Rio Blanco x Idaho 444 population. Therefore, screening the 600+ primer pairs currently available in Dr. Brown-Guedira's laboratory should be sufficient to obtain the desired number of polymorphic markers. Newly-developed primer pairs will not be included in the revised project. One hundred of the polymorphic markers then will be evaluated on 192 of the 320 RI lines to generate a framework molecular map of the population. The budget reduction necessitated reducing the size of the mapping population and the number of markers in the initial map. We will strive to obtain at least two markers per chromosome arm. If the framwork map indicates that specific genomic regions are particularly of interest for dwarf bunt resistance, the remaining lines can be screened with markers for these regions.

Project Team

Brown-Guedira, Gina

Project Annual Reports

FY 2003

Related National Programs

Plant, Microbial & Insect Genetic Res., Genomics, & Genetic Improv. I (301)   Plant Diseases (303)