Project Number: 5325-42000-041-00
Project Type: Appropriated

Start Date: Jul 01, 2001
End Date: May 31, 2006

Objective:
1) develop methods to identify and characterize viral pathogens associated with produce; 2) determine if micro organisms normally present on plants, with phytochemicals, and environmental conditions influence the survival of viruses on/in produce; 3) determine any influence or relationship to growth, for example weeds, on pathogen transfer to produce. FY01 program increase $359,190. Add one SY

Approach:
Model systems for characterizing the attachment and survival of Campylobacter jejuni, E.coli, E.coli 0157:H7, Salmonella and Listeris monocytogens to poultry, fruit and vegatable surfaces will be developed. Mechanisms of attachments will be determined using biochemical , microbiological, immunochemical and biological approached. Anti-pathogen monoclonal antibodies will be produced to detect, monitor pathogens in and on foods, and as inhibitors to detect attachment factors. Characterization of the mechanisms of attachment will provide information for developing new strategies to interfere with attachment. Plant extracts will be screened for compounds involved in attachment and decrease the survival/virulence of pathogens on food products. Methods for measuring the types and viability of pathogens on food surfaces treated w/candidate inhibitors and antimicrobials will be developed including culture enrichment, immuno-capture & cell sorting. Formerly 5325-42000-022-00D (08/01)

Project Team

Mandrell, Robert Fagerquist, Clifton Friedman, Mendel Miller, William Wong, Rosalind Parker, Craig

Project Annual Reports

FY 2003   FY 2002