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Research Project: Evaluation and Enhancement of Small Grains Germplasm for Foliar Disease Resistance

Location: Small Grains and Potato Germplasm Research

Project Number: 5366-21000-022-03
Project Type: Specific C/A

Start Date: Jan 25, 2002
End Date: Dec 31, 2006

Objective:
Evaluate the reaction of USDA-ARS National Small Grains Collection (NSGC) and other small grains germplasm, including both cultivated and wild species, to fungal, bacterial, and viral pathogens, with the ultimate goal of developing enhanced small grains germplasm with improved foliar disease resistance.

Approach:
NSGC accessions or other small grains germplasm will be grown in the field under conditions conducive to disease development and/or greenhouse grown plants will be artificially inoculated. Data obtained from the evaluation trials will be submitted to ARS and entered in the Germplasm Resources Information Network (GRIN) and/or distributed to interested cooperators. The goal of the enhancement phase of the program will be to transfer genes for resistance or tolerance to foliar diseases, frequently found in wild species or other germplasm poorly adapted to commercial production, to germplasm lines or populations for use in cultivar development programs. Documents SCA with University of Minnesota. Formerly 0500-00017-007-08S (09/02).Fine mapping of corn or rice MIPS and low phytic acid 1 loci will use large F2 populations (500 to >1000 individuals) to identify markers closely linked to a given locus. Smaller recombinant inbred line populations will also be used to map new loci and to check map positions of selected clones. Bacterial Artificial Chromosome (BAC) libraries will be screened for those containing selected MIPS or low phytic acid 1 loci. Selected BACs will be sequenced in part (sequencing of selected subclones) or in whole. Corn expression libraries, prepared from selected tissues, will be screened for clones encoding specific kinases. Kinase clones will be mapped using above methods. Formerly 0500-00017-007-08S. Formerly 5366-21000-020-05S (6/03).

 
Project Team
Bockelman, Harold
Brian Steffenson - Assoc. Prof. Plant Pathology (208)397-4162

Project Annual Reports
  FY 2003
  FY 2002

Related National Programs
  Plant, Microbial & Insect Genetic Res., Genomics, & Genetic Improv. I (301)

 
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