Project Number: 5325-21000-011-00
Project Type: Appropriated

Start Date: Jul 24, 2003
End Date: Jul 23, 2008

Objective:
Biotechnologies such as molecular biology, genomics, bioinformatics, and plant transformation will be used to maintain and enhance cereal crop production and quality, with emphasis on wheat and barley. The resources, tools, and knowledge developed will also be applicable, directly or directly, to rye, triticale, and oats, and the other cereal crops. Through collaborative relationships, project efforts will leverage genomics assets into other areas related to crop improvement.

Approach:
1) Apply molecular biology and genetic engineering to wheat high-molecular-weight (HMW) glutenin class seed storage protein genes and other protein classes to understand the molecular bases of their contributions to wheat quality and utilization. Methods will include combinations of molecular alterations, heterologous expression, and plant transformations. 2)Determine the structure and variability of wheat quality-related genetic loci and contribute to the understanding of the organization of the wheat genome. 3) The complement of genes active in wheat seed development will be further developed through expressed-sequence-tags (ESTs). 4) Develop gene expression profiles across wheat seed development and changes in storage protein gene activity and wheat quality. Employ DNA microarrays to profile gene expression during wheat seed development, investigate profile changes during changes in storage protein composition, and will identify candidate genes related to seed traits. Replacing 5325-21430-008-OOD). BSL-1; update pending. Fund transfer $125K from 5325-21000-010-00D; SY time (Yong Gu) changed to 0.6SY (9/2/2003). Previously was 5325-21430-011-00D (10/03).

Project Team

Anderson, Olin L Chingcuanco, Debbie Gu, Yong

Project Annual Reports

FY 2003