Project Number: 500-53-001-90
Project Type: Grant

Start Date: May 13, 2004
End Date: May 12, 2005

Objective:
1. Develop and map more DNA markers in the fine map region containing Qfhs.ndsu-3BS; select more recombinants in Xgwm533-Xgwm493 interval from 1600 F(2) plants, and further narrow down the genomic region containing Qfhs.ndsu-3BS; screen a BAC library and construct a BAC contig spanning the Ofhs.ndsu-3BS. 2. Maintain a germplasm center for Fusarium head blight resistant spring wheat finding additional or new sources of resistance in spring wheat, maintaining and characterizing the resistance and facilitating the utilization of these resistances. 3. Develop near-isogenic lines of major QTLs identified in the resistance sources Fujian 5114 and Wuhan 3; determine the effects of Fusarium head blight resistance QTL derived from Fujian and Wuhan 3 in multiple genetic backgrounds. 4. Screen new putative FHB resistance sources and develop improved spring wheat germplasm containing enhanced levels of FHB resistance; develop Fusarium head blight resistant spring wheat varieties adapted for commercial production in Minnesota and the surrounding region.

Approach:
1. Design primers based on the 3'UTR region, develop CAP and SNP markers; screen another 1600 F(2) from the same NIL population with markers already mapped on the fine mapping population and additional markers to construct a new high resolution map of the region. FHB data of the homozygous recombinants will be used to narrow down the genomic region containing Qfhs.ndsu-3BS; screen homozygous recombinants by point inoculation; assess 40-50 spikes of genotype in each test. Use DNA marker closest to Qfhs.ndsu-3BS to screen a wheat chromosome 3B BAC library. Fingerprint positive BAC clones to begin making a BAC contig of the region, and eventual cloning of Qfhs.ndsu-3BS. 2. Maintain a germplasm center of Fusarium head blight resistant spring wheat utilizing the successful germplasm evaluation and enhancement system. 3. The chromosome 5BL QTL from Fujian 5114 and Wuhan 3, on 5AS and one putatively located on chromosome 1D will be pursued. Genetic mapping in the Fujian 5114 and Wuhan 3 populations is ongoing. QTL-NIL will be developed by recovery of homozygous lines after self pollination of single F(4) or F(5) plants heterozygous for DNA markers flanking the QTL. Test all NIL pairs twice for FHB resistance; represent each genotype by 20-30 inoculated spikes. Test these same materials for FHB resistance in replicated field tests. 4. Make crosses between and among FHB resistance sources and regionally adapted germplasm. Screen field and greenhouse materials to characterize levels of FHB resistance. Test 1,200 lines that are candidates for entry into preliminary field trials for FHB resistance under field conditions. Evaluate 150 lines in advanced yield trials and 100 lines with high levels of FHB resistance in replicated field tests. Test 30-50 lines with high levels of FHB resistance in greenhouse using point inoculation. Release superior germplasm as improved varieties, resistant germplasm.

Project Team

Simmons, Mary - Kay James Anderson - Professor 301-504-5560

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Plant Diseases (303)