Project Number: 5325-42000-027-01
Project Type: Reimbursable

Start Date: Apr 01, 2001
End Date: Sep 30, 2003

Objective:
Develop new methods for cost-effective, rapid testing for pathogens in food. Develop reagents, sample preparation techniques, and specific assay protocols which would be useful for routine surveillance of foods (especially meat and poultry) as well as for use as research tools to study bacteria in food matrices or or on food or equipment surfaces.

Approach:
The project will develop immunoassay and immunosensor technologies, which are based on antibodies as the sensing elements. New methods for sample preparation and analysis will help the food industry and regulatory agencies in the U.S. and Northern Ireland to perform routine surveillance of meat products and to monitor critical control points in food processing to minimize exposure of consumers to diseases caused by foodborne pathogens. Existing antibodies will be utilized and new antibodies developed, as needed. Several different assay formats will be compared for specificity, sensitivity, and robustness. Formats will include enzyme immunoassay, fluorescence immunoassays, and surface plasmon resonance analysis. In addition, immunoaffinity methods will be developed for sample preparation. Characteristics of antibodies that are most suitable for each application will be defined. Documents Reimbursable with FAS. Log 18885.

Project Team

Brandon, David

Project Annual Reports

FY 2003   FY 2002   FY 2001

Related National Programs

Food Safety, (animal and plant products) (108)