NEMI Method Summary

Method Summary Information

Method Number: AG-625 Media: WATER
Revision: Feb-01
Method Source: SYNGENTA
Subcategory: ORGANIC Analytes in this method
Official Name: Atrazine in Drinking Water by Immunoassay
Descriptive Name Atrazine by Immunoassay
Source Info: Syngenta Crop Protection
P.O. Box 18300
Greensboro, NC, 27419
For emergencies, exposure concerns, spills or leaks please call: 1-800-888-8372
For Product Support and Compliance please call: 1-800-334-9481 x2368.
Citation: Atrazine in Drinking Water by Immunoassay
 
Brief Method
Summary:
 Atrazine is detected using a colorimetric immunoassay procedure. A 0.5 mL sample is to a polystyrene culture tube pre-coated with atrazine antibodies. Atrazine in the sample competes with an enzyme conjugate solution for the finite number of antibody binding sites. The tube is washed and the enzyme substrate is added. The substrate is enzymatically converted from a colorless to a blue solution until terminated by acidification. The concentration of atrazine is determined by measuring the absorbance of the solution with a photometer.
Scope And
Application:
 This method determines atrazine in raw and finished drinking water.
Applicable
Conc Range:
 0.05-5.0
Interferences:

(A) Cross-reactivity: Propazine and prometon produce false positive responses for atrazine.

(B) Temperature: Temperature should be kept constant near 22 degrees Celsius for accurate measurements. Avoid drafts and air vents.

(C) Optical interferences: Clean analysis tubes prior to spectroscopic analysis.
QC Requirements: The quality control elements in this method include a method detection limit (MDL) study, an initial precision and recovery (IPR) study, matrix spike/duplicate (MS/MSDs) analyses, and method blank analyses. Also, a full calibration curve is run with each method sample batch.
Sample Handling: Collect 5 mL of sample in a glass container and refrigerate at approximately 4oC. For compliance monitoring samples should be held no longer than 14 days. However, Syngenta performed a study that indicates that the holding time may be extended to two years.
Max Holding Time:
 14 days (for compliance monitoring)
2 years otherwise (see Sample Handling field).
 
Relative Cost: Less than $50
URL to obtain ordering information for this method:
http://www.syngenta.com
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