NEMI Method Summary

Method Summary Information

Method Number: 613 Media: WATER
Revision: 40 CFR Part 136, Appendix A (Current Edition)
Method Source: U.S. EPA Engineering and Analysis Division
Subcategory: ORGANIC Analytes in this method
Official Name: 2,3,7,8-Tetrachlorodibenzo-p-dioxin
Descriptive Name Qualitative confirmation and Quantification of 2,3,7,8-TCDD via GC/MS after a screen using Method 625
Source Info: 4303 USEPA Headquarters
Ariel Rios Building
1200 Pennsylvania Avenue, N. W.
Washington, DC 20460
Citation: EPA Method Guidance CD-ROM (includes MCAWW Methods, and most current EPA Methods)
85438 Byte file
Brief Method
Summary:
 A 1 L sample is spiked with an internal standard of 2,3,7,8-TCDD and extracted with methylene chloride using a separatory funnel. The extract is exchanged to hexane at a concentration of 1.0 mL or less. The extract is then analyzed by capillary column GC/MS to separate and measure 2,3,7,8-TCDD.
Scope And
Application:
 This is a GC/MS method that qualitatively confirms and quantifies the presence of 2,3,7,8-TCDD scanned using Method 625 for municipal and industrial discharges as provided under 40 CFR part 136.1.
Applicable
Conc Range:
 MDL to 1000xMDL (ug/L)
Interferences: (A) Glassware: Must be scrupulously cleaned. Prior to detergent washing, the glassware should be rinsed with the last solvent used and then placed in a muffle furnace to remove any thermally unstable organic compounds. Some thermally organic compounds, such as PCBs may still remain. A rinse with acetone or pesticide quality hexane may be substituted in place of the muffle furnace. Store glassware inverted or capped with aluminum foil.
(B) Co-extraction: The extraction process will sometimes extract certain analytes, such as chlorinated compounds, that may cause possible interference with the target compounds. If these compounds present interference, a cleanup procedure is described in Section 11 of the method.
QC Requirements: Initial demonstration of laboratory capability and the periodic analysis of laboratory reagent blanks, fortified blanks, and other laboratory solutions as a continuing check on performance.
Sample Handling: Samples should be grabbed in 1 Liter or 1 Quart amber bottles with Teflon lined cap. Store samples away from direct sunlight. The bottles must be washed, rinsed with methylene chloride or acetone, and dried before use to minimize contamination. Samples should be iced or kept refrigerated at 4oC from time of collection to time of extraction. If free chlorine is present, add 80 mg of sodium thiosulfate to the sample.
Max Holding Time:
 All samples must be extracted within 7 days of collection and completely analyzed within 40 days if extraction.
Sample Prep Met None.
Relative Cost: $201 to $400
5248
Page 1