NEMI Method Summary


Method Summary Information

Method Number:

D4454

Media:

WATER

Revision:

1985

Method Source:

ASTM International

Subcategory:

MICROBIOLOGICAL

Analytes in this method

Official Name:

Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy

Descriptive Name

Respiring Bacteria in Water

Source Info:

ASTM International
100 Barr Harbor Drive West
Conshohocken, PA 19428
Telephone: (610) 832-9500
Fax: (610) 832-9555
Email: service@astm.org

Citation:

ASTM Volume 11.02

Brief Method
Summary:

A water sample is treated with an aqueous solution of INT-dye (2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride) for 20 min. The reaction then is stopped by adding a 37 % solution of formaldehyde. Sample is filtered through a 0.1-mm pore size polycarbonate membrane filter (presoaked in sudan black solution or equivalent), and stained with acridine orange for 3 min. The filter is then air-dried and examined under oil immersion for total bacteria under epifluorescence illumination and for respiring bacteria under transmitted bright light illumination.

Scope And
Application:

This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to environmental waters and potable waters.

Applicable
Conc Range:

10,000 cells/mL

Interferences:

None indicated.

QC Requirements:

Sample Handling:

Max Holding Time:

Relative Cost:

$51 to $200

URL to obtain ordering information for this method:

http://www.astm.org/cgi-bin/SoftCart.exe/DATABASE.CART/PAGES/D4454.htm?E+mystore

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Method Summary Information