NEMI Method Summary


Method Summary Information

Method Number:

D4455

Media:

WATER

Revision:

1985

Method Source:

ASTM International

Subcategory:

MICROBIOLOGICAL

Analytes in this method

Official Name:

Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure

Descriptive Name

Aquatic Bacteria by Epifluoresence Microscopy

Source Info:

ASTM International
100 Barr Harbor Drive West
Conshohocken, PA 19428
Telephone: (610) 832-9500
Fax: (610) 832-9555
Email: service@astm.org

Citation:

ASTM Volume 11.02

Brief Method
Summary:

Enumeration of aquatic bacteria is obtained by passing a water sample through a 0.2-mm polycarbonate membrane filter. The membrane filter is stained with acridine orange solution. The stained filter is examined for fluorescing bacteria cells using a fluorescent microscope. The fluorescent bacteria are counted. Dilutions are taken into consideration and bacterial concentrations established.

Scope And
Application:

This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters. Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears.

Applicable
Conc Range:

10,000 cells/mL

Interferences:

None indicated.

QC Requirements:

Sample Handling:

Max Holding Time:

Relative Cost:

$51 to $200

URL to obtain ordering information for this method:

http://www.astm.org/cgi-bin/SoftCart.exe/DATABASE.CART/PAGES/D4455.htm?E+mystore

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Method Summary Information