NEMI Method Summary

Method Summary Information

Method Number: 75900 Media: WATER
Revision: 1998
Method Source: Strategic Diagnostics, Inc.
Subcategory: ORGANIC Analytes in this method
Official Name: EnviroGard Endosulfan Plate Kit 75900 (SDI 1998) http://www.sdix.com/
Descriptive Name Endosulfan immunoassay
Source Info: Strategic Diagnostics, Inc.
111 Pencader Dr.
Newark, DE 19702
phone: 302-456-6789
www.sdix.com
Citation: Strategic Diagnostics, Inc.
30364 Byte file
Brief Method
Summary:
 Inhibition immunoassay, with a colorimetric indicator for competitive binding of analyte and an enzyme-labeled conjugate to surface-immobilized antibodies. Colorimetric response is inversely related to the concentration of analyte in the sample or standard. Calibrated to determine endosulfan (mixture of I and II isomers). Reagents are purchased in kits with manufacturers' instructions for sample preparation and analysis. Requirements: a clean water supply to prepare dilutions and wash 96-well plates; filtration equipment to remove particulates from water samples; a 200 rpm orbital mixer for 96-well plates.
A spectrometer which accepts a 96-well plate, for analyses at visible wavelengths (450 nm primary; 600 or 650 nm secondary); equipment to automate sample preparation for large numbers of samples (prefiltration, measurement of uL volumes, plate washing, agitation), so that contact times with reagents are controlled as required; computerized data acquisition and processing. Expendable supplies - approximately $6 per determination.
Scope And
Application:
 Ambient and compliance monitoring.
Applicable
Conc Range:
 0.075 - 1.0 ug/L
Interferences: Sensitivity > endosulfan: endosulfan I; endosulfan II; endosulfan alcohol; endosulfan sulfate; endrin. Sensitivity < endosulfan: dieldrin. Sensitivity << endosulfan: aldrin; chlordane; heptachlor
QC Requirements: Recommended for a kit allowing 96 quantitative determinations: a. calibration with 3 standards and 1 blank, all analyzed in duplicate b. precision: all 44 samples analyzed in duplicate (repeats) c. accuracy: 3 matrix samples, spiked with the target analyte at different levels in the range for quantitative analysis. d. validation: analysis of 4 positive and 4 negative samples by an independent method, for confirmation
Sample Handling: Samples are typically collected in glass containers with Teflon-lined caps. Samples are held in a refrigerator at 4 oC. Immunoassay reagents and kits are stored in the dark at 4 to 8 oC until use.
Water samples generally need to be centrifuged or prefiltered to 0.2 um. Quantitative analysis requires dilution of highly concentrated samples, until they are bracketed by standards in the linear response range. Aliquots of samples and reagents are accurately measured (uL) and mixed for required times, following manufacturer's instructions for use of the materials purchased.
Max Holding Time:
 5 days at 4oC until extraction and analysis
 
Relative Cost: Less than $50
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