NEMI Method Summary


Method Summary Information

Method Number:

9260E

Media:

VARIOUS

Revision:

Standard Methods Online

Method Source:

Standard Methods

Subcategory:

MICROBIOLOGICAL

Analytes in this method

Official Name:

Shigella

Descriptive Name

Qualitative Isolation and Identification of Shigella

Source Info:

Standard Methods Online is jointly produced by the American Public Health Association (APHA), the American Water Works Association (AWWA), and the Water Environment Federation (WEF).
These organizations may be contacted by phone for more information on Standard Methods Online:
APHA: (301) 893-1894
AWWA: (800) 926-7337, (1-303-794-7711, outside U.S. / Canada)
WEF: (800) 666-0206 (1-703-684-2452 outside U.S. / Canada)

Citation:

Standard Methods Online

Byte file

Brief Method
Summary:

The method includes alternative enrichment media to minimuze accumulation of volatile acid by-products derived from growth of potentially antagonistic bacteria. Subsequent concentration by membrane filter or centifugation is recommended depending on sample turbidity. Sequential incubation on two media leads to evalutaion of TSI and LIA slants for reactions that may presumptively identify Shigella spp. Incubation Time: 48 - 72h. Technique: Culture.

Scope And
Application:

Qualitative identification of Shigella in water, soil, sediment, and sludge

Applicable
Conc Range:

N/A

Interferences:

N/A

QC Requirements:

N/A

Sample Handling:

Collect a water sample in a 1L sterile container. Collect soil, sediment, sludge, or other samples in plastic bags or glass or plastic bottles. Maintain samples at 2 - 8 deg. C until they are processed but process them as soon as possible after collection.

Max Holding Time:

N/A

Sample Prep Met

N/A

Relative Cost:

$51 to $200

7129

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Method Summary Information