NEMI Method Summary

Method Summary Information

Method Number: 9510B Media: WATER
Revision: Standard Methods Online
Method Source: Standard Methods
Subcategory: MICROBIOLOGICAL Analytes in this method
Official Name: Virus Concentration from Small Sample Volumes by Adsorption to and Elution from Microporous Filters
Descriptive Name Virus Concentration from Small Sample Volumes by Adsorption to and Elution from Microporous Filters
Source Info: Standard Methods Online is jointly produced by the American Public Health Association (APHA), the American Water Works Association (AWWA), and the Water Environment Federation (WEF).
These organizations may be contacted by phone for more information on Standard Methods Online:
APHA: (301) 893-1894
AWWA: (800) 926-7337, (1-303-794-7711, outside U.S. / Canada)
WEF: (800) 666-0206 (1-703-684-2452 outside U.S. / Canada)
Citation: Standard Methods Online
 
Brief Method
Summary:
 Samples are collected in a sterile container and are processed as soon as possible after collection. The virus-containing sample is pressure-filtered through sterile microporous filters having large surface areas to which viruses adsorb. Viruses are then eluted from the surfaces of the microporous filters by pressure-filtering a small sample of eluent through the filters in situ. Technique: Filtration.
Scope And
Application:
 Concentration of viruses in water
Applicable
Conc Range:
 N/A
Interferences: N/A
QC Requirements: N/A
Sample Handling: Collect sample aseptically in sterile containers. If the sample contains residual chlorine, treat immediately with sodium thiosulfate to a final concentration of 50 mg/L. Do not freeze unless sample cannot be processed in 48 hours; then freeze at -70 deg. C or lower. Sterilize all equipment.
Max Holding Time:
 2 hr. at 25 deg. C or higher; 48 hr. at 2 - 10 deg. C.
Sample Prep Met N/A
Relative Cost: Less than $50
URL to obtain ordering information for this method:
http://www.standardmethods.org/
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