NEMI Method Summary

Method Summary Information

Method Number: 9510E Media: WATER
Revision: Standard Methods Online
Method Source: Standard Methods
Subcategory: MICROBIOLOGICAL Analytes in this method
Official Name: Hydroextraction-Dialysis with Polyethylene Glycol
Descriptive Name Virus Concentration by Hydroextraction-Dialysis with Polyethylene Glycol
Source Info: Standard Methods Online is jointly produced by the American Public Health Association (APHA), the American Water Works Association (AWWA), and the Water Environment Federation (WEF).
These organizations may be contacted by phone for more information on Standard Methods Online:
APHA: (301) 893-1894
AWWA: (800) 926-7337, (1-303-794-7711, outside U.S. / Canada)
WEF: (800) 666-0206 (1-703-684-2452 outside U.S. / Canada)
Citation: Standard Methods Online
 
Brief Method
Summary:
 Polyethylene glycol hydroextraction (PEG) is an ultra-filtration process in which the sample is placed in a cellulose dialysis bag and exposed to PEG, a hygroscopic material. Water and microsolutes leave the sample by passing across the semipermeable dialysis membrane into the PEG. Viruses and other macrosolutes, including PEG, cannot cross the dialysis membrane. The sample volume in the dialysis bag is reduced by water loss to the PEG, thereby concentrating viruses and other macrosolutes. The viruses retained in the dialysis bag are recovered by opening the bag, collecting the remaining sample, and eluting any viruses possibly adsorbed to the inner walls of the bag with a small volume of slightly alkaline proteinaceous solution such as 3% beef extract at pH 9.0. The collected concentrate and eluate are combined and assayed for viruses. Note - only samples of no more than a few hundred milliliters can be processed.
Scope And
Application:
 Concentration of viruses in water
Applicable
Conc Range:
 N/A
Interferences: N/A
QC Requirements: N/A
Sample Handling: N/A
Max Holding Time:
 N/A
Sample Prep Met Sterilize instruments but not the PEG
Relative Cost: $51 to $200
URL to obtain ordering information for this method:
http://www.standardmethods.org/
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