NEMI Method Summary

Method Summary Information

Method Number: 9510F Media: WATER
Revision: Standard Methods Online
Method Source: Standard Methods
Subcategory: MICROBIOLOGICAL Analytes in this method
Official Name: Recovery of Viruses from Suspended Solids in Water and Wastewater
Descriptive Name Virus Recovery from Suspended Solids in Water and Wastewater
Source Info: Standard Methods Online is jointly produced by the American Public Health Association (APHA), the American Water Works Association (AWWA), and the Water Environment Federation (WEF).
These organizations may be contacted by phone for more information on Standard Methods Online:
APHA: (301) 893-1894
AWWA: (800) 926-7337, (1-303-794-7711, outside U.S. / Canada)
WEF: (800) 666-0206 (1-703-684-2452 outside U.S. / Canada)
Citation: Standard Methods Online
 
Brief Method
Summary:
 Viruses in the aquatic environment often are associated with solids or particulate matter, either adsorbed to particulate surfaces or embedded within the solid. Solids-associated viruses may not be eluted efficiently from adsorbent filters or from aluminum hydroxide precipitates and organic flocs. Recovery of solids-associated viruses by microporous filter methods employing in-site elution is inconsistent. Solids associated viruses on adsorbent filters are eluted more efficiently by disrupting filters in elution fluid than by in-situ elution, but this is cumbersome and time-consuming, especially for large diameter disk filters and cartridge filters. For small volumes of water and wastewater, solids-associated viruses can be recovered by separating the solids by centrifuging, decanting the supernatant, and eluting viruses from the solids by resuspending in a small volume of eluent. viruses in the supernatant can by concentrated by one of the procedures described in Standard Methods Sections 9510B, 9510C, 9510D, or 9510E. Viruses eluted from the resuspended solids are separated from the solids by centrifuging and are assayed directly or concentrated further by organic flocculation. Major limits of these methods are incomplete virus elution and poor virus recoveries due to interferences from sample constituents.
Scope And
Application:
 Concentration of viruses in water and wastewater
Applicable
Conc Range:
 N/A
Interferences: Major limitations include incomplete virus elution and poor virus recoveries due to interferences from other constituents in the sample.
QC Requirements: N/A
Sample Handling: N/A
Max Holding Time:
 N/A
Sample Prep Met Sterilize all equipment
Relative Cost: Less than $50
URL to obtain ordering information for this method:
http://www.standardmethods.org/
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