NEMI Method Summary

Method Summary Information

Method Number: 9260B Media: WATER
Revision: Standard Methods Online
Method Source: Standard Methods
Subcategory: MICROBIOLOGICAL Analytes in this method
Official Name: General Qualitative Isolation and Identification Procedures for Salmonella
Descriptive Name General Qualitative Isolation and Identification Procedures for Salmonella
Source Info: Standard Methods Online is jointly produced by the American Public Health Association (APHA), the American Water Works Association (AWWA), and the Water Environment Federation (WEF).
These organizations may be contacted by phone for more information on Standard Methods Online:
APHA: (301) 893-1894
AWWA: (800) 926-7337, (1-303-794-7711, outside U.S. / Canada)
WEF: (800) 666-0206 (1-703-684-2452 outside U.S. / Canada)
Citation: Standard Methods Online
  Byte file
Brief Method
Summary:
 Rather than a specific protocol for Salmonella detection in water, a brief summary of methods suitable for recovery of these organisms is given. Salmonella can be detected at low concentrations in surface waters but may be out-competed by coliforms; therefore it is necessary to analyze a relatively large sample and these samples must first be concentrated. CONCENTRATION TECHNIQUES include: Swab technique (cheesecloth and gauze pads), Diatomaceous earth technique, Large volume sampler, and Membrane filter. Selectively enrich the concentrated sample in a growth medium that suppresses growth of coliform bacteria. No single enrichment medium can be recommended that allows optimum growth of all serotypes, and some modifications can suppress the growth of certain serotypes such as S. typhi. ENRICHMENT TECHNIQUES include: Selenite cystine broth, Selenite-F broth, and Tetrathionate broth. SELECTIVE GROWTH - Separation of pathogens from the remaining nonpathogenic bacterial population is facilitated by proper choice of incubation temperature for primary enrichment and secondary differentiation. All factors in the analysis are interrelated and no one combination is optimum for recovery of all serotypes. Solid media appropriate for detecting enteric pathogens are: Brilliant green agar, bismuth sulfite agar, xylose lysine desoxycholate agar, and xylose lysine brilliant green agar. BIOCHEMICAL REACTIONS: Suspected colonies grown on selective media must be purified and further characterized by biochemical reactions such as: phenylalanine deaminase, TSI, LIA, Urea broth, and fermentation reactions. GENUS IDENTIFICATION: performed using various serological techniques. Technique: Culture.
Scope And
Application:
 Qualitative techniques to isolate and identify Salmonella in water
Applicable
Conc Range:
 N/A
Interferences: N/A
QC Requirements: N/A
Sample Handling: N/A
Max Holding Time:
 N/A
Sample Prep Met N/A
Relative Cost: $51 to $200
7141
Page 1