NEMI Method Summary

Method Summary Information

Method Number: 9260D Media: WATER
Revision: Standard Methods Online
Method Source: Standard Methods
Subcategory: MICROBIOLOGICAL Analytes in this method
Official Name: Quantitative Salmonella Procedures
Descriptive Name Quantitative Salmonella Procedures
Source Info: Standard Methods Online is jointly produced by the American Public Health Association (APHA), the American Water Works Association (AWWA), and the Water Environment Federation (WEF).
These organizations may be contacted by phone for more information on Standard Methods Online:
APHA: (301) 893-1894
AWWA: (800) 926-7337, (1-303-794-7711, outside U.S. / Canada)
WEF: (800) 666-0206 (1-703-684-2452 outside U.S. / Canada)
Citation: Standard Methods Online
  Byte file
Brief Method
Summary:
 Large samples (> 1L) are usually required and concentration by the membrane filter technique (SM-9260B section 1.d) is recommended although other methods are also acceptable. Samples are proportioned into a five-tube, three-dilution set using either selinite cystine, selenite-F, or tetrathionate broth as the selective enrichment medium (SM-9260B section 3). After incubation as necessary, plates from each tube are streaked on plates of brilliant green and xylose lysine desoxychlolate agars followed by 24 h incubation at 35 deg. C. Several colonies suspected of being Salmonella are selected from each plate and a slant each of triple sugar iron and lysine iron agars are inoculated and then incubated for 24 h at 35 deg. C. Test those cultures giving a positive reaction by serological techniques (SM-9260B section 5). The MPN/1.0 L of original sample is calculated from the combination of Salmonella negative and positive tubes. Concentration by the membrane filter technique (SM-9260B section 1.d) is recommended although other methods are also acceptable. Incubation Time: 72 h. Technique: Culture.
Scope And
Application:
 Estimating Salmonella density in water samples
Applicable
Conc Range:
 N/A
Interferences: N/A
QC Requirements: N/A
Sample Handling: N/A
Max Holding Time:
 N/A
Sample Prep Met Membrane filter (SM-9260B  1.d)
Relative Cost: $51 to $200
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