NEMI Method Summary

Method Summary Information

Method Number: 130.31 Media: ANIMAL TISSUE
Revision: March 1998
Method Source: National Oceanic and Atmospheric Administration (NOAA)
Analytes in this method
Subcategory: ORGANIC
Official Name: Gas chromatography mass spectrometry (GC-MS) analysis for organic contaminates in marine animal tissues.
Descriptive Name Organic contaminants in marine animal tissues by GC-MS
Source Info: National Oceanic and Atmospheric Administration (NOAA)
National Status and Trends Program (NS&T;)
SSMC-4, N/SCI-1
1305 East West Highway
Silver Spring, MD 20910
Tel. 301-713-3028
Citation: Sampling and Analytical Methods of the National Status and Trends Program: 1993-1996 Update
990997
Brief Method
Summary:
 Polycyclic aromatic hydrocarbons (PAH), PCDDs, and PCDFs can be determined at low concentrations by mass spectrometry due to their strong molecular ion response. Therefore, GC/MS in the selected ion monitoring mode provides unambiguous and sensitive detection for these compounds. Also the availability of labeled surrogates internal standards of many of the analytes makes very accurate determinations of analyte concentrations possible. Quantitation is performed by gas chromatography mass spectrometry (GC/MS) in the selected ion monitoring (SIM) mode. Silica gel/alumina cleanup with activated copper is used to remove interfering materials from the sample prior to analysis.
Scope And
Application:
 The quantitative method described in this document was used to determine polycyclic aromatic hydrocarbons (PAH) and their alkylated homologues in extracts of marine animal tissue.
Applicable
Conc Range:
 1 - 1000 ng / g
Interferences: Method interferences may be caused by contaminants in solvents, reagents, glassware, and other sample processing hardware, and lead to false positives during instrumental analysis. All materials used in this method are routinely demonstrated to be free from interferences by processing procedural blanks identical to samples (one blank per 20 samples or each batch whichever is more frequent). Matrix interferences result from co-extraction of compounds other than the analytes of interest. Elemental sulfur and naturally occurring lipids can cause interferences in the analysis of tissue extracts.
QC Requirements: The quality assurance / quality control requirements were as follows: Method blank. Laboratory blank spike. Ongoing precision and recovery analyses. Matrix spike. Duplicates. Reference material (NIST SRM 1944) analyses. Labeled compound recovery.
Sample Handling: Tissue is collected and stored in precleaned glass jars and stored frozen (-20 C). Extracts are stored in the dark at or below 4 C.
Max Holding Time:
 6 months (frozen)
Sample Prep Met NOAA TM #130
Relative Cost: Greater than $400
URL to obtain ordering information for this method:
http://nsandt.noaa.gov/publications/download_pdf/techmemo40.pdf
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