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Definition Return to top
Nephelometry is a laboratory technique used to obtain a measurement of the amount of IgM, IgG, and IgA immunoglobulins accurately and rapidly.
The test uses a specialized instrument to measure the movement of particles in a solution (turbidity) caused by the interaction of immunoglobulins in the serum and anti-immunoglobulin that has been added to the serum.
How the test is performed Return to top
Blood is drawn from a vein (venipuncture), usually from the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic, and a tourniquet (an elastic band) or blood pressure cuff is placed around the upper arm to apply pressure and restrict blood flow through the vein. This causes veins below the tourniquet to distend (fill with blood). A needle is inserted into the vein, and the blood is collected in an airtight vial or a syringe. During the procedure, the tourniquet is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.
Infant or young child:
The area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. Cotton or a bandage may be applied to the puncture site if there is any continued bleeding.
How to prepare for the test Return to top
You may be asked to fast for 4 hours before the test.How the test will feel Return to top
When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.Why the test is performed Return to top
The test provides a rapid and accurate measurement of the amounts of immunoglobulins M, G, and A. (Immunoglobulin D has no known clinical significance, and IgE must be measured by more sensitive techniques such as radioimmunoassay or enzyme-linked immunoassay.)Normal Values Return to top
What abnormal results mean Return to top
Increased levels of IgG may indicate the following:What the risks are Return to top
Special considerations Return to top
Nephelometry determines the total amount of each immunoglobulin but cannot distinguish monoclonal antibodies. Other tests such as immunoelectrophoresis or immunofixation can be used to make these distinctions.
Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.
Update Date: 5/8/2003 Updated by: Marcia S. Brose, M.D., Ph.D., Division of Hematology/Oncology, Hospital of the University of Pennsylvania, Philadelphia, PA. Review provided by VeriMed Healthcare Network.
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Page last updated: 28 October 2004 |