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NIH Gene Libraries and Projects

cDNA Libraries

The Mammalian Gene Collection at NIH is constructing cDNA libraries from several human embryonic stem cell lines and will perform expressed sequence tag (EST) sample sequencing from these libraries. This sequencing effort will identify ESTs in the cell lines representing both known and unknown genes. All sequences will be deposited into readily accessible public databases, and the cDNA clones will be accessible through the GenBank database.

These are the current totals for the ESTs deposited in GenBank for the stem cell libraries.

The Cancer Genome Anatomy Project (CGAP)

The goal of the NCI's Cancer Genome Anatomy Project is to determine the gene expression profiles of normal, precancer, and cancer cells, leading eventually to improved detection, diagnosis, and treatment for the patient. By collaborating with scientists worldwide, such as the Ludwig Institute for Cancer Research and Lund University, CGAP seeks to increase its scientific expertise and expand its databases for the benefit of all cancer researchers.

SAGE Genie

Serial Analysis of Gene Expression (SAGE) is a method to count large numbers of mRNA transcripts efficiently by sequencing short tags, usually 10 bp in length. SAGE Genie (Boon et al.) uses a new analytical method of reliably matching SAGE tags to known genes. Based on this tag to gene mapping, the Web site visualizes human gene expression analysis in tissues or individual libraries using displays that are highly intuitive.