|
 |
|
Online Publications
Bacteria
- Method 1605: Aeromonas in
Finished Water by Membrane Filtration Using Ampicillin-Dextrin
Agar with Vancomycin (ADA-V) (October 2001) (443 KB) - This 26-page
method (EPA 821-R-01-034) is presented in Adobe Acrobat® PDF format.
The method describes a performance-based membrane filtration technique
for the detection and enumeration of Aeromonas species. This
method uses a selective medium that partially inhibits the growth of
non-target bacterial species while allowing Aeromonas to grow
and be detected by the production of acid from dextrin fermentation
(causing a pH change producing yellow colonies). The method will be
proposed for use in EPA's data gathering and monitoring programs under
the Unregulated Contaminant Monitoring Rule.
- Method 1604: Total Coliforms and Escherichia
coli in Water by Membrane Filtration Using a Simultaneous Detection
Technique (MI Medium) (September 2002) (486 KB) - This 18-page
document (EPA 821-R-02-024) is presented PDF format. The document is
identical to the February 2000 version of the MI Agar Method (Membrane
Filter Method for the Simultaneous Detection of Total Coliforms and
Escherichia coli in Drinking Water, EPA-600-R-00-13), with one
exception, the addition of MI Broth. The MI Agar Method was approved
for use in compliance monitoring of drinking water and source water
in support of the National Primary and Secondary Drinking Water Regulations
(Federal Register, Vol. 64, No. 230, 1 December 1999, p.67450-67467)
becoming effective 3 January 2000. On 6 November 2001, MI Broth was
approved as a minor modification. This method, now designated as Method
1604, has been approved for use in monitoring ambient water (Federal
Register, Vol. 68, No. 139, 21 July 2003, p.43272-43283) becoming effective
4 August 2003. Four color photos of coliform and E. coli colonies
on MI agar are also included in Method 1604. The procedure is in the
format of the Environmental Monitoring Management Council (EMMC). The
method may also be viewed as it appeared upon 1993 publication in the
journal Applied and Environmental Microbiology
(Vol 59, p.3534-3544), (EPA 600-J-99-225) but it does not include
photos (2.2MB).
- Method 1600: Enterococci in Water by Membrane
Filtration Using membrane-Enterococcus Indoxyl-ß-D-Glucoside Agar
(mEI) (Septermber 2002) (125 KB) - This 14-page method (EPA 821-R-02-022)
is in PDF format. It combines information previously published in the
May 1997 version of Method 1600 (Membrane Filter Test Method for Enterococci
in Water, EPA-821-R-97-004a) and a subsequent March 2000 manual (Improved
Enumeration Methods for the Recreational Water Quality Indicators: Enterococci
and Escherichia coli, EPA/821/R-97/004). Method 1600 is a revision
of EPA's previous enterococci method, used since 1985 in ambient water
quality monitoring. It reduces analysis time to 24 hours and improves
analytical quality. The method has been validated in single- and multi-laboratory
studies and has undergone peer review.
- Method 1106.1: Enterococci in Water by Membrane
Filtration Using membrane-Enterococcus-Esculin Iron Agar (mE-EIA) (Septermber
2002) (806 KB) - This 16-page method (EPA 821-R-02-021) is in PDF
format. It combines information from a 1985 publication (Test methods
for Escherichia coli and Enterococci in Water by the Membrane
Filter Procedure, EPA 600-4-85-076) and a subsequent March 2000 manual
(Improved Enumeration Methods for the Recreational Water Quality Indicators:
Enterococci and Escherichia coli, EPA/821/R-97/004). Method 1106.1
is a membrane filter (MF) procedure for the detection and enumeration
of enterococci in water. The method incorporates the sequential use
of a selective medium, mE agar, and a differential medium, EIA agar.
- Method 1103.1: Escherichia coli (E. coli)
in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia
coli Agar (mTEC) (Septermber 2002) (471 KB) - This 17-page method
(EPA 821-R-02-020) is in PDF format. It combines information from a
1985 publication (Test methods for Escherichia coli and Enterococci
in Water by the Membrane Filter Procedure, EPA 600-4-85-076) and a subsequent
March 2000 manual (Improved Enumeration Methods for the Recreational
Water Quality Indicators: Enterococci and Escherichia coli, EPA/821/R-97/004).
Method 1106.1 is a membrane filter (MF) procedure for the detection
and enumeration of E. coli in water. The method incorporates
the use of a selective and differential medium, mTEC agar followed by
incubation with urea substrate medium.
Protozoans
- Method 1623: Cryptosporidium
and Giardia in Water by Filtration/IMS/FA - April 2001
Update (1.2 MB) - This 58-page document (EPA 821-R-01-025) is PDF format.
Method 1623 is a performance-based method applicable to the determination
of Cryptosporidium and Giardia in aqueous matrices.
Method 1623 requires filtration, immunomagnetic separation (IMS) of
the oocysts and cysts from the material captured, and immunofluorescent
assay (FA) for determination of the oocyst and cyst concentrations.
- Method 1622: Cryptosporidium
in Water by Filtration/IMS/FA - April 2001 Update(1.2 MB) - This
57-page document (EPA 821-R-01-026) is in PDF format . It is a
performance-based method applicable to the determination of Cryptosporidium
in aqueous matrices. Method 1622 requires filtration, immunomagnetic
separation (IMS) of the oocysts from the material captured, and immunofluorescent
assay (FA) for determination of the oocyst concentration. There are
also forms associated with Method 1622.
Viruses and Coliphage
- USEPA Manual of Methods
for Virology - Twelve chapters. The individual chapters were prepared
as ASCII text files to be viewed/printed with a monospaced font. It
is planned to make them also available in PDF format in the future.
The manual (EPA 600-4-84-013) describes in detail procedures for detecting
viruses in environmental samples.
| Recreational Water Publications |
- Health Effects Criteria for Marine Recreational Waters - This 98-page
document is in PDF format. This 1983 report (EPA 600-1-80-031)
presents health effects water quality criteria for marine recreational
waters. It can be used to develop guidelines based upon acceptable rather
than detectable risk and is consistent with risk analysis. The
document has been split into sections for easier downloading.
Part 1 (1.2 MB), Part
2 (1.2 MB), Appendix Part 1 (782KB),
App Part 2 (1.1 MB), App
Part 3 (1.1 MB), App Part 4 (1.1
MB), App Part 5 (789 KB).
- Health Effects Criteria for Fresh Recreational
Waters (1.3 MB) - This 33-page document is in PDF format.
This 1984 report (EPA 600-1-84-004) provides an assessment of the relationship
between microbiological indicators of water quality and swimming-associated
illness. The report resulted from a series of EPA-supported epidemiological-microbiological
research projects designed to develop risk-based recreational water
criteria for fresh waters.
Note: Sample collection for the Information Collection Rule ended
in December, 1998. The following ICR publications are maintained online
as informational resources.
- ICR - Microbial Laboratory Manual (1.2MB)
- This 233-page document (EPA 600-R-95-178) is presented in PDF format.
This is the "ICR Microbial Laboratory Manual" referenced in
the Federal Register. It describes in detail the microbiolgical techniques
used with the ICR.
- ICR - MPNV Software Program (168 KB)
- This program was used to perform the virus analyses for the ICR -
calculating MPN (Most Probable Number) and confidence limits values
(see the Virus Quantitation section, page VIII-27 of the ICR Microbial
Laboratory Manual). This program is a DOS program. Save the file to
a directory of your choice and (in DOS) type "MPNV" followed
by " Enter." The file can also be run as a DOS application
under Microsoft Windows® . See Example 1(page VIII-48 to VIII-55) and
Example II (page VIII-57 to VIII-62) for examples of how to use the
program.
Note: Adobe Acrobat Reader is required to view PDF documents. The
most recent version of the Adobe Acrobat Reader
is available as a free download. An Adobe Acrobat
plug-in for assisted technologies is also available.
|
